Figure S2. MTT assay result of GH3 cells interfaced with nanowire-grown substrates in various densities (PS: plane substrate, LDSN, MDSN and HDSN: nanowire-grown substrate shown in Figure 1a, 1b and 1c). Figure S3. SEM images of primary hippocampal neurons cultured on nanowire-grown substrates in order of Figure 1a, 1b and 1c. A white circle in d indicates
penetrated nanowire from bottom to top membrane of neuron. Figure Selleck Ku-0059436 S4. (a) A schematic drawing for observation of cell/nanowire interface. Dotted line represents a sectioning direction of FIB. Square part is the area we observed by SEM (b) SEM images of primary hippocampal neurons-nanowire interface (N: nanowire, P: platinum layer for the protection of upper part of cell, C: cell soma). Arrow indicates cell membrane, which is covered by gold layer for a first SEM observation. Figure S5. Cyclic voltammogram of individual nanoelectrode in 0.1 M K3Fe(CN)6. Ag/AgCl electrode was served as the reference electrode and a platinum wire was served as the auxiliary electrode. The scan rate was 10 mV/s. Figure S6. Equivalent circuit of our measurement system (Cm: cell membrane capacitance, Em: cell membrane potential, Rm: cell membrane resistance, Rleak: junction leakage resistance, Re: electrode resistance, Ce: electrode capacitance). (DOCX 4 MB) References 1. Hamill OP, Marty A, Neher E: Improved patch-clamp techniques for
high-resolution current Fedratinib cell line recording from cells and cell-free membrane patches. Pflug Arch Eur J Phy 1981, 391:85–100.CrossRef MAPK Inhibitor Library concentration 2. Markram H, Lübke J, Frotscher M, Sakmann B: Regulation of synaptic efficacy by coincidence of postsynaptic APs and EPSPs. Science 1997, 275:213–215.CrossRef 3. Marom S, Shahaf G: Development, learning and memory in large random networks of cortical neurons: lessons beyond anatomy. Q Rev Biophys 2002,35(1):63–87. 4. Stuart G, Spruston N, Sakmann B, Häusser M: Action potential initiation and backpropagation in neurons of the mammalian CNS. Trends Neurosci 1997,20(3):125–131.CrossRef 5. Bean BP: The action potential in mammalian central neurons. Nat Rev Neurosci 2007, 8:451–465.CrossRef 6. Fromherz P: Electrical interfacing
of nerve cells and semiconductor chips. Chem Phys Chem 2002,3(3):276–284.CrossRef 7. Eschermann JF, Stockmann R, Hueske M, Vu XT, Ingebrandt S, Offenhäusser A: C1GALT1 Action potentials of HL-1 cells recorded with silicon nanowire transistors. Appl Phys Lett 2009, 95:083703.CrossRef 8. Gabay T, Jakobs E, Ben-Jacob E, Hanein Y: Engineered self-organization of neural networks using carbon nanotube clusters. Physica A 2005, 350:611–621.CrossRef 9. Zheng B, Hsieh S, Wu CC, Wu CH, Lin PY, Hsieh CW, Li IT, Huang YS, Wang HM, Hsieh S: Hepatocarcinoma single cell migration on micropatterned PDMS substrates. Nano Biomed Eng 2011, 3:99–106. 10. Bi GQ, Poo MM: Synaptic modifications in cultured hippocampal neurons: dependence on spike timing, synaptic strength, and postsynaptic cell type. J Neurosci 1998, 18:10464–10472. 11.