Furthermore, CNN1 and TAGLN were down regulated in the intestinal proteome in Lean fish. Collagen, the main component of connective tissue, helps to maintain the structural integrity of tissues, while osteonectin is an extracellular matrix glycoprotein with high affinity towards collagen and whose expression has been associated sellekchem with remodelling processes in tis sues, including human intestine during development morphogenesis Inhibitors,Modulators,Libraries and in diseased mucosa. Troponin, TAGLN and CNN1 are all involved in actin binding, actin myosin interaction and muscle contraction. The inverse regulation of troponins is not conflicting as they have different roles in actomyosin cross bridge forma tion and contraction, binding of troponin C to Ca2 induces conformational changes that counteract the in hibitory action of troponin I.
Expression of TAGLN transcript and protein showed Inhibitors,Modulators,Libraries opposite effects but a lack of correlation between transcriptomic and proteomic data is not unprecedented. Inhibitors,Modulators,Libraries As discussed above, this result might also be explained by the presence of similar duplicated genes in Atlantic salmon that are regulated differently. Transcriptomic results were validated by RT qPCR for COL1A2, although only significantly when fish were fed the VO diet, for which fold changes were higher. In addition, in the microarray results differences in expression of structural proteins between family groups were consistently more accentuated in fish fed VO which could suggest a cumulative effect of diet.
Fur thermore, MYO was up regulated in fish fed VO com pared to FO but only in Lean fish, and significant diet �� genotype interactions were found for alpha actinin 1, tubulin beta 2 chain and procollagen lysine 2 oxoglutarate 5 dioxygenase 2, which were up regulated in Lean salmon, Inhibitors,Modulators,Libraries compared to Fat, but only when fed VO. In cod, replacement of FO by VO resulted in changes in intestinal expression of structural genes with the potential to alter the structural and mechanical properties of the intestinal muscle layer, including a range of actin binding transcripts. The present study is the first investigation of the influ ence of genetic background of families with different flesh adiposity phenotypes on intestinal Inhibitors,Modulators,Libraries gene expression of a fish species. Effects were subtle and consequently their potential impacts difficult to fully assess.
However, if genetic selection for families better adapted to alterna tive formulations is an approach taken in the future, the potential for genotype specific differences being exacer bated when VO replaces dietary FO should be further examined to assess the consequences of these changes in intestinal gene expression. Conclusions Metabolic activity, particularly lipid and energy, of intes Regorafenib IC50 tinal tissue responded to dietary lipid composition but was also affected by genotype.