We tested the impact of DJ-1 expression on overall survival. The results showed that the overall survival time was significantly
dependent on DJ-1 expression, pT status, and UICC stage. Discussion The current TNM staging and histopathological grading systems are useful prognostic indicators for SSCC . However, they have limitations with regard to providing APR-246 datasheet critical information regarding patient prognosis. Patients with the same clinical stage and/or pathological grade of SSCC often display considerable variability in disease recurrence and survival [1, 28]. Therefore, new objective measures and biomarkers are necessary to effectively differentiating patients with favorable outcomes from those with less favorable outcomes. Molecular biomarkers
in conjunction with standard TNM and histopathological strategies have the potential to PI3K inhibitor predict prognoses more effectively. DJ-1 protein is coded by exons 27, contains 189 amino MK-1775 solubility dmso acids, and weights about 20 kD, and was firstly defined as an oncogene candidate in 1997 . Recent studies showed that DJ-1 is expressed highly in many types of human malignancies [2, 5–15]. Lines of evidence have also suggested that the over-expression of DJ-1 is correlated with more aggressive clinical behaviors of pancreatic, esophageal and lung cancers [10–13]. However, in our recent glottic squamous cell Reverse transcriptase carcinoma study , DJ-1 has only been identified as a prognostic marker and activator of cell proliferation, and the expression of DJ-1 was not correlated to clinical lymph node metastasis. This non-invasive role of DJ-1 in glottic squamous cell carcinoma which is contradictory to the invasive role of DJ-1 in other malignancies may be attributed to the clinical and biological
behavior of glottic squamous cell carcinoma, as this type of LSCC was poorly invaded in clinic. So, in order to identify whether DJ-1 also play the invasive role in LSCC, SSCC, the more aggressive type of LSCC, was selected in the present study. Recently, several studies showed that PTEN in human malignancies is associated with cell proliferation, tumor invasion, and TNM stage, and can be down-regulated by DJ-1 in several cancers, such as renal cancer, breast cancer, bladder cancer, and ovarian cancer [8, 24–26]. In 2005, Kim RH  found that DJ-1 could activate cell proliferation and transformation by negatively regulating PTEN expression in breast cancer cells. In 2012, Lee H  showed that over-expression of DJ-1 and loss of PTEN are associated with invasive urothelial carcinoma of urinary bladder. Taken together, we hypothesized that DJ-1 would promote migration and invasion of SSCC via down-regulating the expression of PTEN, and may associated with clinical lymph node status in SSCC.