Radiosensitivity, DNA damage, and cell cycle measurements were made using standard clonogenic assays, immunofluorescent assessment of nuclear histone H2AX phosphorylated on serine-139 (gamma-H2AX) foci, and flow cytometry. Western immunoblotting and fluorescence confocal microscopy were used to analyse
the changes in cellular proteins. Real-time polymerase chain reaction (PCR) was used to compare levels of aurora A mRNA.
Results: Compared to C33aV cells, C33aE6 cells showed enhanced radiation cell killing. This was associated with a large amount of polyploidy which was followed by late cell death in C33aE6 cells. Aurora A was highly expressed in C33aE6 cells at pre- and post-irradiation times compared to C33aV cells. Silencing aurora A resulted in a reduced amount of residual gamma-H2AX foci in C33aE6 MI-503 Epigenetics inhibitor cells, and diminished the difference in radiosensitivity between the C33aE6 3-deazaneplanocin A chemical structure and C33aV cells.
Conclusion: Our in vitro results indicate that genetic instability could be augmented in the HPV-infected cancer cells by up-regulation of aurora
A, especially against a background of dysfunctional p53. Further studies are needed to examine whether aurora A could be a viable therapeutic target in HPV-related tumours.”
“We present a general theoretical approach that combines the iterative technique with the nonlinear transfer matrix method to solve the general problems of three-wave mixing with pump depletion in nonlinear multilayer structures. The theoretical model is directly derived from nonlinear coupled wave equations, shows fast numerical convergence and maintains conservation of total energy perfectly for general structures. We have employed this approach to discuss
second harmonic generation in photonic bandgap crystals with slow light effect and obtained a conversion efficiency of about 25% for both the forward and backward second harmonic waves. The detailed wave evolution process and energy conversion mechanism of sum frequency generation and difference frequency generation in perfect and imperfectly quasiphase-matching structures have been systematically investigated and their subtle and strong dependence on the initial conditions of incident waves has been revealed. The developed theoretical approach can be very useful for efficient and quantitative Vorinostat analysis of various nonlinear optical problems and help to design optimum nonlinear multilayer structures for specific high-efficiency nonlinear three-wave mixing processes. (C) 2011 American Institute of Physics. [doi:10.1063/1.3556739]“
“Purpose: To identify genes responsible for the radiosensitivity, we investigated the role of the differential gene expression profiles by comparing radioresistant H1299 with radiosensitive H460 lung cancer cell lines.
Materials and methods: mRNA profiles of lung cancer cell lines were assessed using microarray, and subsequent validation was performed with qRT-PCR (Quantitative real time-polymerase chain reaction).