Figure 6b shows current of working electrode without phenyl hydrazine and with 100.0 μL phenyl hydrazine. It is obvious that the addition of phenyl Rabusertib hydrazine enhances electrical current which suggests that composite nanorods are sensitive to phenyl hydrazine. Thus by insertion of phenyl hydrazine, augmentation in electrical current implies that nanorods has fast and susceptible response to the phenyl hydrazine. The rapid electron

swap and good electro-catalytic oxidation properties are accountable for the high electrical response of composite nanorods to phenyl hydrazine [7–9]. Figure 6 I-V characterization of composite nanorods. (a) Current comparison of composite nanorods coated and un-coated Au, (b) comparison of coated electrode current with and without phenyl hydrazine, (c) concentration variation of phenyl hydrazine, and (d) calibration plot. Phenyl find more hydrazines easily undergo catalytic dissociation reaction by applying to I-V technique and generate diazenyl benzene, 2H+, and

2e– which cause increase in electrical conductivity [10, 11]. Generally, electron emission takes place from the chemisorbed oxygen into the conduction band of the sensor and ionizes atmospheric oxygen molecules by giving electron from the conduction band and ionosorbed on the surface as Oads − (O− or O2 − depending on the energy available). The resulting equation is (1) The surface adsorbed oxygen Selleckchem GW3965 (Oads −) reacts with diazenyl benzene produced by the catalytic reaction of phenyl hydrazine and produce benzenediazonium ion (Figure 7) [12–15]. Figure 7 Mechanism of phenyl hydrazine in the presence of composite nanorods. The electrical

response of phenyl hydrazine was studied in the concentration assortment of 5.0 μM to 0.01 M by consecutive addition into 0.1 M PBS solution with constant stirring, and the outcomes are given away in Figure 6c. The results show increase in electrical current is directly proportional to the concentration of phenyl hydrazine which increased with increase in concentration of phenyl hydrazine. The gradual increase in current suggests that the number of ions increases with increase in phenyl hydrazine concentration by giving extra electron to the conduction band of composite nanorods [16, 17]. The N-acetylglucosamine-1-phosphate transferase calibration curve was plot out from the current variation and is depicted in Figure 6d. The calibration curve indicates that at first, current raises with rise in phenyl hydrazine concentration but behind definite concentration, the current turns into constant which reflects saturation at this specific concentration. The lower part of the calibration curve is linear with correlation coefficient (R) of 0.8942, while the slope of this linear lower part gave sensitivity which is 1.5823 μA.cm−2.μM−1. Composite nanorods displayed linear dynamic range from 5.0 μM to 1.0 mM and detection limit of 0.5 μM.