To our knowledge the only comparable data set avail able is from human transformed germinal centre B cells which have been cultivated on a CD40L expressing feeder cell line for 24 hours. Regardless of the various experimental disorders, BL2 cells showed comparable gene expression changes after publicity to recombinant CD40L for 6 hours. In con trast, international gene expression adjustments following B cell receptor activation, for BAFF, LPS or IL21 stimulation are actually described making use of various microarray platforms. Therefore, a quantitative comparison is challenging. In addition, differ ent cell lines or leukocyte cell subsets from a various ori gin, such as splenic murine B cells or bursal chicken B cells have been analysed. A choice of readily available information is sum marized in Further file eight, Supplemental 1.
Gene set enrichment analyses of worldwide gene expression changes in transformed germinal centre B cells Molecular functions, biological processes, cellular com ponents and pathways Trichostatin A structure affected by distinct stimuli were characterized by gene ontology based gene set en Staurosporine richment analyses. IgM activated genes are linked to MAP kinase activ ity, phosphatase activity and transmembrane transporter action. The biological processes affected is usually sum marized as regulation of immune responses, MAP kinase action, and programmed cell death, regulation of meta bolic processes or cell cycle and pressure responses. IL21 activated genes are enriched for gene sets connected with responses to virus together with other organisms and cytokine manufacturing such as kind I interferon biosynthetic professional cesses.
Additionally, as for IgM activated genes, IL21 impacted gene sets are involved in regulation of professional grammed cell death. The involvement of IL21 activated genes on cytokine signalling could also describe its relation to I?B kinase/NF ?B cascade and NF ?B import into nu cleus, gene sets characteristic for Toll like receptor path means, Jak/STAT and chemokine signalling pathway, but additionally pathways in cancer are enriched. IL21 suppressed genes are characteristic for nucleotidyltransferase action, cytoskeletal protein or phospholipid binding so affecting cell shape, morphogenesis or chemotaxis. BAFF activated genes are involved with metabolic processes of amino acids and chromatin remodelling, whereas downregulated genes are part of lipoprotein metabolic method, protein amino acid acylation. The CD40L mediated gene expression adjustments positively impact MHC class I receptor action and therefore antigen pro cessing and presentation of peptide antigen, the regulation of membrane potential, compact GTPase mediated signal transduction also as metabolic processes. In contrast, CD40L suppressed genes are involved in phospholipase ac tivity or unfavorable regulation of transcription.