The uptake of radiolabeled gomesin by each organ was calculated u

The uptake of radiolabeled gomesin by each organ was calculated using the following equation: DI% = (CPM organ/standard CPM) × 100), where%DI = percentage of the injected dose and CPM = count per min [35]. Statistical analysis ANOVA, with the post-Tukey test, was used to evaluate the statistical significance of results obtained in all experiments except the blood and survival analysis, where the Students t-test and Log-rank test were used, respectively. The differences between the results obtained with treatment compared to the controls were considered statistically significant when

the p value was less than 0.05. Acknowledgements We are grateful to Susana P. Lima for technical assistance and Cassiano Pereira for figure preparation. This work was supported by Brazilian grants: Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) and the Conselho learn more Nacional de Desenvolvimento Científico e Tecnológico (CNPq). AG-014699 research buy References 1. Bulet P, Stocklin R, Menin L: Anti-microbial peptides: from invertebrates to vertebrates. Immunol Rev 2004, 198:169–184.PubMedCrossRef 2. Brogden KA: Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nat Rev Microbiol 2005,3(3):238–250.PubMedCrossRef 3. Mookherjee N, Hancock RE: Cationic host defence peptides: innate immune regulatory peptides as a novel approach for treating infections. Cell Mol Life Sci 2007,64(7–8):922–933.PubMedCrossRef

4. Silva PI Jr, Daffre S, Bulet P: Isolation and characterization of gomesin, an 18-residue cysteine-rich defense peptide from the spider Acanthoscurria

gomesiana hemocytes with sequence similarities to horseshoe crab antimicrobial peptides of the tachyplesin family. J Biol Chem 2000,275(43):33464–33470.PubMedCrossRef 5. Mandard N, Bulet P, Caille A, Daffre S, Vovelle F: The solution structure of gomesin, an antimicrobial cysteine-rich peptide from the spider. Eur J Biochem 2002,269(4):1190–1198.PubMedCrossRef Methane monooxygenase 6. Fazio MA, Oliveira VX Jr, Bulet P, Miranda MT, Daffre S, Miranda A: Structure-activity relationship studies of gomesin: importance of the disulfide bridges for conformation, bioactivities, and serum stability. Biopolymers 2006,84(2):205–218.PubMedCrossRef 7. Barbosa FM, Daffre S, Maldonado RA, Miranda A, Nimrichter L, Rodrigues ML: Gomesin, a peptide produced by the spider Acanthoscurria gomesiana , is a potent anticryptococcal agent that acts in synergism with fluconazole. FEMS Microbiol Lett 2007,274(2):279–286.PubMedCrossRef 8. Miranda A, Miranda MTM, Jouvensal L, Vovelle F, Bulet P, Daffre S: Animal toxins. In Gomesin: a Powerful Antimicrobial Peptide Isolated from the Brazilian Tarantula Spider Acanthoscurria gomesiana Edited by: KERALA. 2008. 9. Rodrigues EG, Dobroff AS, Cavarsan CF, Paschoalin T, Nimrichter L, Mortara RA, Santos EL, Fazio MA, Miranda A, Daffre S, et al.: Effective topical treatment of subcutaneous murine B16F10-Nex2 melanoma by the antimicrobial peptide gomesin. Neoplasia 2008,10(1):61–68.PubMedCrossRef 10.

Comments are closed.