The TonB2 protein was verified to be immunogenic and could afford partial protection for animals from lethal infection. In the present study, the recombinant TonB2 (rTonB2) was overexpressed in Escherichia coli wBL21(DE3) and purified. The rTonB2 was then used as antigen to immunize BALB/c mice for the production of monoclonal antibodies (MAb). Four clones of TonB2-specific MAb secretion hybridomas-2F2, 2G8, 3D2, and 6F10-were selected. The MAbs 2F2, 3D2, and 6F10 were classified as IgG1 isotype and 2G8 was of IgG2a isotype. Western blot and ELISA results indicated that MAbs had specific binding activity to rTonB2. The MAbs generated here will
be used for further functional analyses of the TonB2
protein.”
“Objectives: EVP4593 The objective was to evaluate the impact of gender on long-term survival of patients who underwent non-cardiac vascular surgery.
Design, Material and Methods: Our prospectively collected Selleck LBH589 data contained information on 560 patients undergoing carotid endarterectomy (CEA), 923 elective abdominal aortic aneurysm repairs (AM) and 1046 lower limb reconstructions (LLR). Patient characteristics and long-term mortality of women were compared to that of men. Kaplan-Meier (KM) survival curves were constructed for men and women, on which we superimposed age- and sex-matched KM survival curves of the general population. Cox proportional hazards regression was used to identify risk factors for mortality.
Results: Men in the CEA group had statistically significant higher all-cause mortality, hazard rate ratio (HRR) 1.41(95% CI 1.01-1.98) No differences in mortality between the genders were observed in the AAA and LLR groups.
Overall, men had more co-morbidities but received more disease-specific medication compared to women. Women retained their higher life expectancy after CEA but lost it in the AAA and LLR groups.
Conclusion: Women retain their higher life expectancy after CEA; however, after AAA repair and LLR, this advantage is lost.
Both men and women received too little disease-specific medication, but women were worse off. (C) 2011 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.”
“Rubber seed oil (Hevea LY333531 chemical structure Brasiliensis) a non-edible oil, widely grown in India with fatty acid composition, palmitic, 9.3%; stearic, 8.4%; oleic, 25.4%; linoleic, 41.1% and linolenic, 15.3% acids, was exploited for the preparation of lubricant base stocks. The oil was hydrolyzed to obtain fatty acids and these fatty acids were reacted with branched chain alcohol, 2-ethyl-1-hexanol (2-EtH) and polyols namely neopentyl glycol (NPG), trimethylolpropane (TMP), pentaerythritol (PE) at 135-140 degrees C in the presence of para toluene sulfonic acid (p-TSA) as catalyst in xylene medium. The branched and polyol esters were obtained in the range of 91-96.5% yields.