The strain carrying PmglB-gfp was grown in chemostats (at D = 0.15 h-1, with 5.6 mM Glc) and analyzed with flow cytometry. A) For subsequent https://www.selleckchem.com/products/citarinostat-acy-241.html analysis,

the cells were gated using the autogating tool (FlowJo, Tree Star, Inc.) in the densest area of the pseudo-color plots of SSC vs. FSC. B) The gating was performed 24 times to capture between 5,000-20,000 cells, and the resulting distributions of GFP fluorescence were plotted. This yielded mean log expression of 2.69 ± 0.005 (mean ± standard deviation) and CV was 0.13 ± 0.0014. This suggests that the results for mean expression and CV deviated less than 1% when gate size was varying 4-fold. Our gate size varied maximally selleck compound 1.2-fold when analyzing 10,000-12,000 cells, therefore the slight differences in the gate size should minimally influence the computation of mean and CV. (TIFF 681 KB) References 1. Davidson CJ, Surette MG: Individuality in Bacteria. Annu Rev Genet 2008, 42:253–268.PubMedCrossRef 2. Veening JW, Smits WK, Kuipers OP: Bistability, epigenetics, and bet-hedging in bacteria. Annu Rev Microbiol 2008, 62:193–210.PubMedCrossRef 3. Elowitz

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