The results are Venetoclax concentration shown in Table 2. Since these concentrations were genotoxic, new concentrations were tested in order to find concentrations that did not induce genotoxic damages (0.5 μg/mL, 0.1 μg/mL, 0.05 μg/mL, 0.01 μg/mL, 1 ng/mL, 100 pg/mL and 10 pg/mL). From the results obtained, it was observed

that the concentrations of 1 ng/mL, 100 pg/mL and 10 pg/mL were not statistically significant in relation to the negative control. Thus, these three concentrations were used in the assessments of the antigenotoxic potential of the wasp venom. The data concerning the antigenotoxic evaluation are shown in Table 3. By the results observed, none of the concentrations tested (1 ng/mL, 100 pg/mL and 10 pg/mL) was effectively able to decrease and/or inhibit the genotoxic action of MMS. The same concentrations used in the comet assay were also used to evaluate the mutagenicity of the wasp venom (10 μg/mL,

5 μg/mL, 1 μg/mL, 0.5 μg/mL, 0.1 μg/mL, 0.05 μg/mL, 0.01 μg/mL, 1 ng/mL, 100 pg/mL and 10 pg/mL). The results are shown in Table 4. As for the genotoxic damages, the concentrations of 1 ng/mL, 100 pg/mL and 10 pg/mL were not statistically significant in relation to the negative control. Thus, these three concentrations were selected to be used in the evaluations of the antimutagenic potential of the wasp venom (Table 5). Likewise in the antigenotoxicity assay, none of the concentrations tested was able to inhibit and/or decrease the mutagenicity induced by MMS, therefore, they were not see more considered good antimutagenic agents. Venoms of social wasps are rich in biogenic

amines, biologically active peptides and proteins (Lorenzi, 2002 and Nakajima et al., 1986). Among these substances it can be highlighted the phospholipases, hyaluronidases and mastoparans. In the present study it was observed that concentrations above 10 μg/mL are able to induce death of Carnitine palmitoyltransferase II the HepG2 cells, and the concentration of 80 μg/mL was capable of inducing the death of approximately 50% of the cells. We highlight, therefore, that it is very difficult to occur exposure to this concentration, since in a single sting of vespids it can be injected into the skin only about 20 μg of the venom. This concentration can, according to Reisman and Livingston (1992), be enough to trigger the sensitization process in human beings. However, from our results the concentration of 20 μg/mL did not induce high cytotoxicity for the exposed cells. Our results also showed that, although concentrations lower than 17 μg (10 μg/mL, 5 μg/mL, 1 μg/mL, 0.5 μg/mL, 0.1 μg/mL, 0.05 μg/mL, 0.01 μg/mL) had not induced cytotoxicity for the HepG2 cells, they present a genotoxic and mutagenic potential for these cells. This capacity may have been triggered as a result of the action of several proteins present in the venom on the cell membrane, which can lead to an alteration in the permeability of these cellular structures.