Introduction of the endoscope into the sub-mucosal space was easily achieved without need for electrosurgical

dissection. The scope appeared to have a piston effect by pushing the gel distally resulting in further dissection by the gel. In essence, the submucosal lifting gel created a tunnel by “auto-dissection” find more of the submucosal layer. The myotomy is performed by careful dissection of sling fibers at the cardia of the stomach. The incision was performed across the circular muscular layers.The dissection was gradually and carefully lengthened and deepened to the level of the longitudinal fibers.After successful myotomy, the entrance was closed using endoclips. This animal case demonstrates that using the Submucosal Dabrafenib mouse Lifting Gel for POEM procedures has some potential benefits; 1.The submucosal lifting gel appears to “Auto dissect” which would decrease the need for electrosurgical dissection using a knife

or needle, 2.The gel appears to have a tamponade effect, thereby minimizing bleeding, 3.The transparency of the gel allows excellent visibility of the submucosal space. “
“Secondary stricture formation is the major drawback for resections >3 cm or more than 75% of the esophageal circumference at esophageal ESD. In March 2011 we embarked on animal experiments regarding esophageal resection and re-transplantation of esophageal and gastric mucosal patches in pigs under an approved protocol (NLVL No: 33-42502-06/1151) for stricture prevention. CASE REPORT: A 72 y old man with swallowing difficulty (DG1); tabacco use of 20 py until >15 y ago. Prior rectal resection with sigma anus praeter for a T2 distal rectal cancer. EGD: Suspicion of early squamous cell cancer (Paris IIa; EUS UT1a, m, N0), >75% circum-ferential tumor spread within the cervical esophagus and upper sphincter area (17-25 cm aborally). Biopsy: SC HG-IEN. On April 13, 2011 we performed an EGD under general anesthesia with tracheal

intubation with first tubular ESD Diflunisal over 10 cm from the lower hypopharynx through the UES from 17-27 cm followed by a 9×4 cm ESD in the gastric antrum. The healthy gastric specimen retrieved was cut longitudinally into 3 mucosal stripes that were attached to the denuded esophageal muscular layer by means of hemoclips. The stripes were gently pressed against the wall by a non-covered self-expanding metal stent with the intent to allow also a luminal nutrition of the specimen. The sphincter area of 1.5 cm length had to be spared. The esophageal specimen showed a non-invasive low horny early squamous cell cancer (pT1a G2 L-, V-) and curative resection (R0; invasion depth of lamina propria max. 150 microns). Stent removal was performed at day 20 and was cumbersome due to local mucosal hyperplasia. However, multiple islets of gastric mucosa had successfully grown at the esophageal resection site. The patient was discharged on day 24 and regularly seen as outpatient.