Although none of the SNPs caused a significant effect on steady-state levels of NAT1-specific mRNA, C97T(R33stop), C190T(R64W), C559T (R187stop) and A752T(D251V) each reduced NAT1 protein level and/ or N-and O-acetyltransferase catalytic activities to levels below detection. G560A(R187Q) substantially GNS-1480 reduced NAT1 protein level and catalytic activities and increased substrate Km. The G445A(V149I), G459A(synonymous) and T640G(S214A) haplotype present in NAT1* 11 significantly (P<0.05) increased NAT1 protein level and catalytic activity. Neither T21G(synonymous), T402C(synonymous),
A613G(M205V), T777C(synonymous), G781A(E261K) nor A787G(1263V) significantly affected Km, catalytic activity, mRNA or protein level. These results suggest heterogeneity among slow NAT1 acetylator phenotypes.”
“2-Chlorohexadecanal (2-ClHDA), a 16-carbon chain chlorinated
fatty aldehyde that is produced by reactive chlorinating species attack of plasmalogens, is elevated in atherosclerotic plaques, infarcted myocardium, and activated leukocytes. We tested the hypothesis that 2-ClHDA and its metabolites, 2-chlorohexadecanoic acid (2-ClHA) and 2-chlorohexadecanol (2-ClHOH), induce COX-2 expression in human coronary artery endothelial cells (HCAEC). COX-2 protein expression increased in response to 2-ClHDA treatments at 8 and 20 h. 2-ClHA also increased COX-2 expression following an 8 h treatment. Quantitative PCR showed that 2-ClHDA treatment increased COX-2 mRNA over 8 h, while 2-ClHA treatment led to a modest increase PI3K inhibitor by 1 h and those levels remained Selleckchem PCI-34051 constant over 8 h. 2-ClHDA led to a significant increase in 6-keto-PGF(1 alpha) release (a measure of PGI(2) release) by HCAEC. These data suggest that 2-ClHDA and its metabolite 2-ClHA, which are produced during leukocyte activation, may alter vascular
endothelial cell function by upregulation of COX-2 expression.”
“Nasopharyngeal mucoepidermoid carcinoma (MEC) is an extremely rare entity. To date, there is little published about its clinical characteristics and treatment outcomes. Between 1997 and 2009, 13 cases of MEC were confirmed and treated at the department of Radiation Oncology, Cancer Hospital of Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC). Nasal obstruction, bleeding and hearing loss were the most common presentations, whereas, neck mass, headache and cranial nerve palsy were uncommon. Tumors remained stable after either primary radiation therapy or post-operative radiation therapy for the residual, though the majority of them were high or high-intermediate grade tumors. Five patients, who received either primary surgery or salvage surgery, had positive surgical margins, however, all are alive with stable disease except one old patient died of heart failure.