8%(4/41) vs 41.7%(5/12),P < 0.05). No serious complications were encountered in both groups, such as pleural effusion, mediastinitis and digestive tract fistula. Conclusion: POEM with transverse entry incision can significantly decrease operation duration and reduce incidence of pneumatosis-related complication, while relieving symptoms dramatically. Key Word(s): 1. Achalasia; 2. POEM; 3. Transverse incision; Presenting Author: MENG LI Additional Authors: BIN

LU, LI CHU, HONG ZHOU, MINGYAN CHEN Corresponding Author: MENG LI, BIN LU Affiliations: YAP-TEAD Inhibitor 1 First Affiliated Hospital of Zhejiang Chinese Medical University Objective: Epidemiological studies suggest that uninvestigated dyspepsia (UD) is common. However, there is little data on the prevalence of UD and its overlap with other gastrointestinal diseases in the college student population. The aim of this survey was to investigate UD in college students in the Zhejiang province. Methods: A total of 2520 college students completed a questionnaire. The diagnosis of UD and irritable bowel syndrome (IBS) was based on the Rome-III criteria. Gastroesophageal reflux disease (GERD) was defined as episodes of heartburn

and/or acid regurgitation that occurred at least once a week. Results: A total of 1870 students (967 males, mean age 21.34 years) completed the questionnaire. The incidence of UD was higher in females and in senior students. The GSK3235025 in vitro prevalence of UD, IBS, GERD, UD + GERD overlap and UD + IBS overlap was 108 (5.67%), 129 (6.89%), 17(0.91%), 12 (0.64%) and 18 (0.96%), respectively. Conclusion: The incidence of UD is relatively low in Chinese college students. Overlap between UD and IBS or GERD is common, suggesting the involvement of common pathophysiological disturbances. Key Word(s): 1. college student; 2. Dyspepsia; 3. gastroesophageal; 4. prevalence; Presenting Author: HE DE-ZHIHE DE-ZHI MIDDLE NAME: Additional Authors: LI JIAN-SHENGLI JIAN-SHENG LI JIAN-SHENG Corresponding Author: HE DE-ZHIHE DE-ZHI MIDDLE NAME: Affiliations: Zhengzhou University Objective: to investigate the expression of IGFBP7 in gastric cancer and its

clinical MCE significance. Methods: Real-time quantitative PCR and Western blotting were employed to examine the expression of IGFBP7 in 73 paired normal gastric mucosa and adenocarcinoma tissues at mRNA and protein levels respectively. Another 281 paraffin-embedded gastric cancer blocks were used for immunohisochemical detection for IGFBP7 expression. The correlation of IGFBP7 expression and clinicopathological parameters and postoperational survival in gastric cancer was further explored by statistical analyses. Results: IGFBP7 expression was significantly reduced at both the mRNA (P < 0.001) and the protein (P < 0.001) levels in gastric cancer tissues. Immunohistochemical analysis revealed that IGFBP7 expression was completely lost in 169 out of the 281 (60.1%) patient samples.