Tract-specific analysis by using white matter tractograms enables more precise measurements and better anatomical localization of white matter. The purpose of this study was to investigate the use of MK to estimate changes in the spinal cord, separately for white matter and

gray matter, in patients with early cervical spondylosis. Veliparib in vivo Thirteen consecutive patients diagnosed with cervical myelopathy by clinical signs and symptoms participated in this study. Their demographic characteristics are summarized in Table 1. Prior to the study, the research protocol was approved by the institutional review board, and informed consent was obtained from each patient. The exclusion criteria were as follows: the presence of other intraspinal diseases such as tumors, a history of neck surgery for any disease, or unsatisfactory image quality for calculating diffusion metrics. All images were acquired on a 3 T MR scanner (Achieva; Philips Medical Systems, Best, The Netherlands). The imaging parameters for DKI were as follows: repetition time/echo time, 10758/88 ms; number of excitations, two; slice thickness/gap, 4/0 mm; GSK1349572 supplier number of slices, 32; field of view, 64 × 64 mm; matrix, 128 × 128 reconstructed; imaging time, approximately 13 min; and

four b-values (0, 700, 1400, and 2100 s/mm2) with diffusion encoding in 6 directions for each b-value. The gradient length (δ) and time between the two leading edges of the diffusion gradient (Δ) were 9.8 and 44.1 ms, respectively. A reduced field-of-view technique was used to improve image quality [19] and [20]. Before DKI, conventional turbo spin-echo T1- and T2-weighted sagittal and axial images

were obtained. The imaging parameters for sagittal images were as follows: repetition time/echo time, 400/10 ms for T1-weighted imaging (T1WI) and 3246/128 ms for T2-weighted imaging (T2WI); echo train length, 4 for T1WI and 36 for T2WI; number of excitations, two; slice thickness/gap, 3/0.3 mm; number of slices, 11; field of view, 250 × 250 mm; and matrix, 512 × 512. Imaging parameters for the axial images were as follows: repetition time/echo time, 726/10 ms for T1WI and 6196/93 ms for T2WI; echo train length, 5 for T1WI and 36 for T2WI; number of excitations, two; slice thickness/gap, 4/0.4 mm; number of slices, 24; field of view, 160 × 160 mm; and matrix, 512 × 512. Analyses of DTI, tractography, and Celecoxib DKI were performed by using the free software dTV II FZRx and Volume-One 1.81 (Image Computing and Analysis Laboratory, Department of Radiology, The University of Tokyo Hospital, Tokyo, Japan) [21] on an independent Windows PC. First, maps for FA, ADC, and MK were calculated. The FA and ADC maps were established on the basis of a conventional mono-exponential model that assumes a Gaussian probability diffusion function, by using data at b-values of 0 and 700 s/mm2. Next, we performed diffusion tensor tractography of the bilateral lateral funiculus with threshold values for the termination of fiber tracking set to FA > 0.