9% (197,242; fresh oocytes versus FVO P < 0.001) of inseminated oocytes generated Selleck WH-4-023 good embryos. Implantation rate using FVO was 6.9%, which was significantly lower than that using

fresh oocytes (13.5%; P < 0.001) and frozen embryos (8.8%; P < 0.001). Pregnancy rate per transfer using FVO was 12.5% and significantly lower than that using fresh oocytes (24.9%; P < 0.001) or frozen embryos (16.4%; P < 0.001). There were 505 deliveries after IVF with FVO and 582 babies. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.”
“Background: Listeria monocytogenes usually causes mild maternal illness, but can be devastating to the fetus. Case: Listeriosis in a pregnant patient successfully treated with empiric antibiotic therapy is described. After induced delivery, despite severe placental

infection, a healthy infant was born. Additionally, archived placental specimens with similar pathological manifestation were evaluated for evidence of L. monocytogenes. Conclusion: It is important to emphasize the value of associating maternal data and clinical and laboratory findings of the newborn with the pathologic placental findings to get better results in the etiologic diagnosis of the listeriosis.”
“This study assessed the effects of vitrification solutions and equilibration times on morphology of cynomolgus ovarian tissues. Ovarian cortical sections (0.1-0.2 cm thickness) of seven cynomolgus monkeys were randomly allocated to either a control group or one of six vitrification

groups. Ovarian tissue sections were vitrified ultra-rapidly by placing them find more directly into liquid nitrogen using two different vitrification solutions (VSEGP: 5.64 mol/l ethylene glycol + 5% (w/v) polyvinylpyrrolidone + 0.5 mol/l sucrose; and VSED: 3.22 mol/l ethylene glycol + 2.56 mol/l dimethylsulphoxide + 0.5 mol/l sucrose) after three different exposure times (5-20 min). After warming, follicle morphology was analysed using light and transmission electron microscopy. The proportion of morphologically normal follicles vitrified using VSED after a 5-min exposure was lower (P < 0.05) Bromosporine cell line than those vitrified by other conditions. The proportion of normally structured mitochondria in oocytes of preantral follicles vitrified after a 5-min exposure to VSED (56%) was lower (P < 0.01) than those vitrified by other conditions (78-88%). Following tissue vitrification with VSED, the surface ratio of lysosome was increased compared with non-vitrified oocytes (1.64% versus 1.11%; P < 0.05). These results indicate that VSEGP can support the morphology of vitrified preantral follicles and oocytes. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.”
“Routine early developmental parameters are widely used in IVF centres to evaluate embryo development and fresh single-blastocyst transfer currently seems superior to single-embryo transfer.