bovis typing patterns (TPs) other than the dominant A1 and B2 did not differ statistically from 1998-2003 to 2006-2007 (2.2 ± 4.3% in 1998-2003, 9.3 ± 5.5% in 2006-2007, Chi-square = 2.39, 1 d.f., n.s., confidence limits are calculated according to Sterne’s exact method). No spoligotyping patterns other than

the two dominant ones (A and B) were detected among 47 cattle isolates in 2006 and 2007. Changes in mycobacterial typing buy GDC-0973 patterns over time in DNP All three M. bovis typing patterns recorded in DNP wildlife between 1998 and 2003 (A1, B2, C1) were still evidenced in similar proportions in 2006-2007 (Chi-square = 0.5, 2 d.f., n.s.). However, while only three different TPs had been detected in DNP wildlife in the first period, up to 8 different ones were found in the second period (Table 3). Two of these “”new”" TPs (D4 and F1) had already been recorded in cattle sampled in DNP between 1998 and 2003. However, 3 other TPs (A3, B5, and E1) had

never before been reported in DNP. Table 4 Spoligotyping patterns of Mycobacterium bovis isolates from Doñana cattle, by zone. Zone A B Marisma de Hinojos (Large, N to S ranging Marshland) 7 3 Los Sotos (SO) 7 2 El Puntal (PU) 5 5 Las Nuevas (Southern Marshland, close to selleck MA and PU) 6 3 Zone not known 7 2 Total 32 15 In contrast with the situation in wildlife and to data from 1998-2003, when 10 out of 41 cattle spoligotyping Astemizole patterns were different from A and B, no spoligotyping patterns other than the two dominant ones (A and B, Table 4) were detected among 47 cattle isolates in 2006 and 2007 (Chi-square = 12.9, 3 d.f., p < 0.001). Table 5 Czechanovsky similarities (in %) (from north to south, CR Coto del Rey; SO Los Sotos; EB Estación Biológica; PU El Puntal; MA Marismillas) and host species (WB wild boar; RD red deer; FD fallow deer) in DNP.   CR SO EB PU MA WB RD FD CR - 50 36 40 20 57 62 54 SO   - 55 60 40 57 62 91 EB     - 89 67 77 67 60 PU       - 75 67 73 67 MA         - 67 54 44 WB           - 53 61 RD             - 50 FD               - Spatial

structure Regarding the MOTT (Table 1, Figures 4 and 5), M. interjectum was only found in wild boar from EB, in the central part of DNP. In contrast, M. scrofulaceum was found in all three wildlife hosts (but not in cattle) in CR (2 isolates), SO (18), EB (5), and PU (3). The only MOTT found in cattle (one M. intracellulare isolate) was isolated from a cow raised in PU. M. intracellulare was often isolated from wild boar in PU and EB, and also from one fallow deer in EB and two red deer in SO and MA, respectively. Figure 4 Spatial structure of Mycobacteria Other Than Tuberculosis (MOTT) and Mycobacterium bovis isolates from wild ungulates in Doñana National Park, Spain. MOTT were proportionally more frequent in the central parts of the park (SO, EB, PU; see Figure 6).