The distribution of these LSPs was thus

investigated Selleckchem PF-6463922 across our representative panel of Map S-type selleck chemicals llc strains from various origins. As shown in Figure 1, analysis by PCR supports the association of the LSPA20 region with C-type strains whereas the LSPA4 region is present in all S-type strains. Presence of the LSPA4 region was not related to PFGE subtype I versus III, of the country of origin and pigmentation status (Table 1). Figure 1 Detection of types and subtypes of strains based on of the absence or presence of large sequences LSPA4 (A) and LSPA20 (B) investigated by PCR. SNP analysis Since SNPs found in gyrA and B genes have been reported to be subtype (I, II, III)-specific, the panel of Map S-type strains was subjected to SNP analysis and compared to C type K-10 strain. As shown in Table 3, consensus sequences obtained matched those previously published and distinguished types I, II and III of Map. Table 3 SNPs found in gyrA and gyrB genes for M. avium subsp. paratuberculosis strain K-10 and M. avium subsp. paratuberculosis types I and III Strains Type IS900 RFLP profiles gyrA gyrB position 1822 1986 1353 1626 K10* II R01 CCCGAGGAGCGGATCGCT- ACTCGTGGGCGCGGTGTTGT selleck screening library CCGGTCGACCGATCCGCGC- CCAGCACATCTCGACGCTGT

6756 I S1 …..A….- ………. ……C…- ………. 6759 I S1 …..A….- ………. ……C…- ………. P133/79 I S2 …..A….- ………. ……C…- ………. 21P I S2 …..A….- ………. ……C…- Selleckchem Rucaparib ………. 235 G I S2 …..A….- ………. ……C…- ………. M189 I S2 …..A….- ………. ……C…- ………. M15/04 I S2 …..A….- ………. ……C…- ………. M254/04 I S2 …..A….- ………. ……C…- ………. M71/03 I S2 …..A….- ………. ……C…- ………. M72/03 I S2 …..A….- ………. ……C…- ………. 22 G III A …..A….- …..T….. ……C…- …..T….. OVICAP16 III A …..A….- …..T….. ……C…- …..T…..

OVICAP49 III A …..A….- …..T….. ……C…- …..T….. 21I III B …..A….- …..T….. ……C…- …..T….. PCR311 III B …..A….- …..T….. ……C…- …..T….. 19I III C …..A….- …..T….. ……C…- …..T….. 85/14 III C …..A….- …..T….. ……C…- …..T….. OVICAP34 III D …..A….- …..T….. ……C…- …..T….. 18I III E …..A….- …..T….. ……C…- …..T….. FO21 III F …..A….- …..T….. ……C…- …..T….. LN20 III I1 …..A….- …..T….. ……C…- …..T….. 269OV III I10 …..A….- …..T….. ……C…- …..T….. M284/08 III I10 …..A….- …..T….. ……C…- …..T….. P465 III I2 …..A….- …..T….. ……C…- …..T…..