Scleroramularia henaniensis G.Y. Sun, H.Y. Li & Crous, sp. nov. Fig. 7
Fig. 7 Scleroramularia henaniensis (CPC 18167). A. Colony on malt extract agar. B. fragmenting conidia in older cultures on synthetic nutrient-poor agar. C. Two conidia joined by hyphal bridge (anastomosis). D–H. Disarticulating conidial chains. Scale bars = 10 μm MycoBank MB517456. Etymology: Named after its type locality, Henan Province, China. Scleroramulariae asiminae morphologice valde similis, sed conidiis brevioribus; conidiis basalibus, anguste cylindraceis, 1–3-septatis, 22–70 × 1.5–2 μm; conidiis intercalaribus et terminalibus anguste ellipsoideis vel fusoidibus-ellipsoideis, 0–3-septatis, (7–)12–17(–20) × (1.5–)2(–2.5) μm. On selleckchem SNA. Mycelium creeping, superficial and submerged, consisting of hyaline, smooth, branched, septate, 1–2 μm diam hyphae.
Conidiophores mostly reduced to conidiogenous cells, or with one supporting cell. Conidiogenous cells solitary, erect, intercalary on hyphae, subcylindrical, straight, with 1–2 terminal loci, rarely with a lateral locus, 2–5 × 2–3 μm; scars thickened, darkened and somewhat refractive, 0.5–1 μm wide. Conidia in branched chains, hyaline, smooth, finely guttulate, straight or gently curved if long and thin; basal conidia mostly narrowly cylindrical, 1–3-septate, 22–70 × 1.5–2 μm; intercalary and terminal conidia becoming more narrowly ellipsoid Proteasome activity to fusoid-ellipsoid, 0–3-septate, (7–)12–17(–20) × (1.5–)2(–2.5) μm; hila thickened, darkened and somewhat refractive, 0.5–1 μm wide. Culture characteristics: After 2 weeks at 25°C sporulating profusely on SNA, white with abundant aerial mycelium, reaching 20 mm diam. On OA flattened, spreading, with sparse aerial mycelium, and even, raised margins, white, reaching 20 mm diam. On MEA spreading, flattened, with sparse aerial mycelium,
surface white, ridged, with feathery margin; ITF2357 mouse reverse umber in middle, orange to sienna in outer region, reaching 15 mm diam; surface white, reverse umber in centre and outer region. On PDA flattened, spreading, with moderate aerial mycelium, and feathery margin; much surface cream to white, reverse umber in middle, sienna in outer region, reaching 20 mm diam after 2 weeks. Black, globose bodies (sclerotia), variable in size, are sparsely formed on MEA and PDA. Appearance on apple: Compact speck consisting of shiny, black, flattened sclerotium-like bodies, round to irregular (35–418 μm diam) appressed to the cuticle and densely arranged (5–22/mm2) with irregular margins. Specimen examined: CHINA, Henan Province, Lingbao, on fruit surface of apple cv. ‘Fuji’, 6. Oct. 2006, H. Li, CBS H-20481 holotype, ex-type cultures CPC 18167 = 06-LHY-HNIb-8 = CBS 128073. USA, Kentucky, on fruit surface of apple cv. ‘Golden Delicious’, Sept. 2005, P. Tokosh, CPC 16104 = KY238B1a = CBS 128074; USA, New York, on fruit surface of apple cv. ‘Gold Rush’, Oct. 2005, D. Rosenberger, CPC 16106 = NY2CS4b = CBS 128075.