Light intensity, 1,120 μmol m−2 s−1. Selleckchem FG 4592 Attached
dandelion leaf. 5 ms light/dark intervals. a Plots of the two signals versus CO2 concentration for 2.1 and 21 % O2. b Relationship between the rates of CO2 uptake and charge flux as a function of CO2 concentration in three different dandelion leaves at 2.1 % O2. The symbols represent black diamonds, leaf 1, 5 ms light/dark; black filled circles, leaf 1, 10 ms light/dark; red triangles, leaf 2, 5 ms light/dark; blue squares, leaf 3, 5 ms light/dark. Maximal charge flux and CO2 uptake signals were normalized Figure 9b summarizes the relationship between the rates of CO2 uptake and charge flux in the presence of 2.1 % O2 as a function of CO2 concentration as derived from three independent measurements using different leaves and in one case also a different
modulation frequency of actinic light (light/dark periods selleck inhibitor of 10 ms instead of 5 ms). While at high CO2 the relationship is close to linear, it becomes curvi-linear at lower CO2, with CO2 uptake distinctly declining relative to P515 indicated charge flux. This finding agrees with the notion that alternative types of electron transport, like the MAP-cycle (Schreiber and Neubauer 1990; Schreiber et al. 1995), also called water–water cycle (Asada 1999; Miyake 2010), or cyclic PS I (Heber and Walker 1992; Joliot and Joliot 2002, 2005; Joliot and Johnson 2011) are stimulated when electron flow to CO2 becomes limited by lack of CO2. However,
in spite of the low O2 concentration present in the experiments of Fig. 9b, also some CRT0066101 in vitro stimulation of oxygenation (photorespiration) may occur at low CO2 concentration. Simultaneously measured oscillations of CO2 uptake, P515, and charge Molecular motor flux Oscillations in photosynthetic parameters have been demonstrated in numerous previous studies and have been discussed in terms of largely differing mechanisms (Sivak and Walker 1986; Furbank and Foyer 1986; Peterson et al. 1988; Stitt and Schreiber 1988; Laisk et al. 1991, 1992; Siebke and Weis 1995; Joet et al. 2001; Nedbal and Brezina 2002). As regulatory oscillations can be observed best in intact leaves, investigations aiming at unraveling their mechanism have been relying primarily on non-invasive indicator signals like Chl fluorescence, light scattering and P700 absorbance at 810–830 nm, measured simultaneously with O2 evolution or CO2 uptake. In the discussion of the obtained data, apparent phase shifts between the various signals have played a central role. Damped oscillations in CO2 uptake can be induced by sudden increases of CO2 or O2 concentration. Simultaneous measurements of such oscillations in CO2 uptake, P515 and P515 indicated charge flux are presented in Fig. 10. Fig.