Otherwise, 14 patients would be enrolled in stage 2. If 2 or fewer total responses were observed, then the null hypothesis would be accepted. Else, the third stage would accrue to a total of 40 etc patients. If 4 or more responses were observed among 40 patients, then the drug would be considered efficacious. This procedure had a power of 0. 91 and a significance level of 0. 10. The probability of early ter mination was approximately 0. 85 under the null hypoth esis. For the correlative assays, descriptive statistics were proposed to describe changes in post treatment versus pre treatment specimens. Tumor biopsies and assays for FT activity and RAS pathway signaling Excisional biopsies were performed to obtain sufficient tis sue for analysis and to minimize sampling error.

Tissue was rapidly processed and stored until batched analysis. Proteins were extracted Inhibitors,Modulators,Libraries from snap frozen tumor tissue using a tissue protein extraction reagent from Pierce. After homogenization at 4 C, the samples were spun at 13,000 x g and the supernatant found between Inhibitors,Modulators,Libraries the fatty top layer and the pellet was used for biochemical analysis. The FTase enzymatic assays as well as Western blots for protein level determination were carried out as described previously. All analyses were performed in the laboratory of Dr. Said Sebti, at Moffitt Cancer Center. Measurement of FTI action on T cells ex vivo Peripheral blood mononuclear cells were Inhibitors,Modulators,Libraries separated from heparinized blood samples and stored as viable cells in freezing medium until batch analysis.

Briefly, cells were thawed, cultured with the superantigen Staphylococcal enterotoxin A or with Phorbol Inhibitors,Modulators,Libraries Myristate Acetate Ionomycin as a positive control, with or without the addition of R115777 in vitro as a comparison. After over night culture, supernatants were analyzed for IFN con tent by ELISA using antibody pairs from Pharmingen. Post versus pre treatment samples were compared using a paired t test. In parallel, cells were lysed and analyzed by Western blotting for the apparent molecular weight of the farnesylated protein HDJ 2 as described previously. Results Patient characteristics Fourteen patients with metastatic melanoma were en rolled in this study between May 2003 and April 2005. The median age was 56 years, and 9 were male. Five patients reported prior immuno therapy Inhibitors,Modulators,Libraries for metastatic disease, and 7 had an elevated LDH.

Toxicity and clinical response Treatment with R115777 was generally well tolerated. Only two patients showed grade 3 toxicities. selleck chemicals llc One patient experienced grade 3 nausea and vomiting, which was associated with an increased serum BUN. A second pa tient experienced grade 3 myelosuppression and anorexia. These adverse events were readily reversible. Table 1. Clinical response was assessed using RECIST criteria. There were no objective partial or complete responses observed in this cohort of 14 patients.