Intra-day and inter-day studies [Table selleck compound 2] support the precision of the method. The proposed method when used for estimation of THIO and ACE from the pharmaceutical dosage form after spiking with the working standard afforded recovery of 99�C101% [Table 3]. Table 2 Intra-day and inter-day precision (n = 3) Table 3 Results of the accuracy study Sensitivity of the ethod (LOD and LOQ) The limit of detection was found to be 20 ng/spot and 10 ng/spot, while the limit of quantitation was found to be 60 ng/spot and 30 ng/spot for LOR and THIO, respectively. The low value of LOD and LOQ indicates that the method is sensitive. Robustness The standard deviation of the peak areas was calculated for each parameter change and the % RSD was found to be less than 2%. The low values of the % RSD [Table 4] indicated robustness of the method.

Table 4 Robustness study of lornoxicam and thiocolchicoside (n = 3) Specificity The method was found to be specific as no interfering spots were seen when Rf values of the standard and sample were compared. There is no difference in the spectra of the sample and standard solution, which indicate the specificity of the method. The peak purity of both drugs was assessed by comparing the respective spectra of standard drugs and samples at peak start, peak apex and peak end positions of the spot. CONCLUSION HPTLC, with its advantage of low-operating cost, high-sample thought and minimum sample preparation need, is now-a-days preferred as a routine analytical technique for control and assurance.

The validated HPTLC method employed here proved to be simple, fast, accurate, precise and sensitive and, thus, can be used for routine analysis of LOR and THIO in tablet dosage form to determine uniformity of contents for both the analytes in tablet in a short time. ACKNOWLEDGMENT The authors are thankful to m/s Glenmark Pharmaceuticals Ltd., Baddi, India and Medley Pharmaceuticals Ltd., Baddi, India for providing gift samples of LOR and THIO, respectively. The authors are thankful to the Management of MAEER’s Maharashtra Institute of Pharmacy, Pune and Anchrom Laboratories for providing necessary facilities to carry out the research work. Footnotes Source of Support: Nil Conflict of Interest: None declared.

Meta-cresol (m-cresol) is used as bactericide in the biotechnological processing of pharmaceuticals; preservative in pharmaceutical formulations [injection solutions of insulin, somatropin, and parathyroid hormone AV-951 (PTH)]; pesticide for the treatment of the stems of fruit trees and plants. Exposure of humans is possible through the use of m-cresol as a preservative in pharmaceutical injection solutions. Meta-cresol, para-cresol, and m/p-cresol mixtures are absorbed across the respiratory and gastrointestinal tracts and through the skin, and are distributed throughout the body.