All participants signed an informed consent form that 17-DMAG fda explains the testing and training procedures conducted during the study. The study protocol was approved by the ��Research Ethics Committee of Rio de Janeiro Federal University (Brazil)��. Data collection pre and post-training (10 weeks) was performed in four days. On the first visit, between 7:00 and 8:00 am, anthropometric and flexibility measures were made. On the second day (24 hours after), all the tests were repeated to determine the test retest reliability. On the third day (24 hours after), the 5 repetitions maximum test (5RM) was applied. On the fourth day, 48 hours later, the 5RM test was repeated. 5 Repetitions Maximum Test (5RM) The subjects were evaluated in two non-consecutive days in both pre and post training.

The 5RM test was conducted for the exercises: bench press (BP) and leg press (LP). All exercises were performed on machines Rotech Fitness? (Goiania – Brazil). To minimize the error during the 5RM tests, the following strategies were adopted (Sim?o et al., 2005): (a) standardized instructions concerning the testing procedures were given to participants before the test; (b) participants received standardized instructions on exercise technique; (c) the exercise technique of subjects was monitored and corrected as needed during testing, because variations in the positioning of the joints involved in the movement could activate other muscles, leading to misinterpretation of scores, and; (d) standard verbal encouragement was provided during the test procedure.

During the 5RM test, each subject had a maximum of three attempts at each exercise with a rest interval of 5 minutes between them and 10 minutes before the start of the test of the next exercise. The standard exercise technique was conducted for each exercise. No pause was allowed between the eccentric and concentric phases of a repetition nor between the repetitions. The range of motion determined should be achieved to define completion of a successful repetition. The heaviest load achieved on either of the test days was considered the 5RM. Flexibility Measurement (Sit and Reach Test) Flexibility was measured before and after 10 weeks using a Sit and Reach Test (ACSM, 2000). The subject sat with their feet firmly against the testing box, keeping their knees extended and hands placed one over the other; reached forward, sliding the hands along the measuring ruler.

The considered score was the greatest distance recorded in the three attempts with a 10-second interval between them (ACSM, 2000). The same procedure was performed after training. GSK-3 All flexibility tests were conducted in the same period of the day. Data collected during the first evaluation were not available to the examiner to prevent information bias during the measurements taken after training. Before the flexibility test, a warm-up of 4 stretching exercises was performed for the muscle groups involved in the evaluation.